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Practical Protocol for Making Calibration Curves for Direct and Sensitive Quantitative LC Orbitrap-MS of Large Neuropeptides

Peptides larger than 3–4 kDa, such as neuropeptide Y (NPY), orexin-B, and alpha-MSH, have practical issues that arise when conducting direct and sensitive quantitative liquid chromatography (LC) orbitrap-FT mass spectrometry (MS) due to their adsorption and low ionization efficiency, especially in s...

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Detalles Bibliográficos
Autores principales: Yamagaki, Tohru, Yamazaki, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Mass Spectrometry Society of Japan 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392841/
https://www.ncbi.nlm.nih.gov/pubmed/32802701
http://dx.doi.org/10.5702/massspectrometry.A0087