Fluorescence-assisted sequential insertion of transgenes (FASIT): an approach for increasing specific productivity in mammalian cells

Currently, the generation of cell lines for the production of recombinant proteins has the limitation of unstable gene expression due to the repeat-induced gene silencing or the loss of transgene copies resulting from recombination events. In this work, we developed a new strategy based on the seque...

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Detalles Bibliográficos
Autores principales: Bravo, Felipe E., Parra, Natalie C., Camacho, Frank, Acosta, Jannel, González, Alaín, Toledo, Jorge R., Sanchez, Oliberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392891/
https://www.ncbi.nlm.nih.gov/pubmed/32732973
http://dx.doi.org/10.1038/s41598-020-69709-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392891/
https://www.ncbi.nlm.nih.gov/pubmed/32732973
http://dx.doi.org/10.1038/s41598-020-69709-1

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