Pyroptotic cell death in the R6/2 mouse model of Huntington’s disease: new insight on the inflammasome

Mechanisms of tissue damage in Huntington’s disease involve excitotoxicity, mitochondrial damage, and neuroinflammation, including microglia activation. In the present study, we investigate the role of pyroptosis process in the striatal neurons of the R6/2 mouse model of Huntington’s disease. Transg...

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Autores principales: Paldino, Emanuela, D’Angelo, Vincenza, Sancesario, Giuseppe, Fusco, Francesca R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395807/
https://www.ncbi.nlm.nih.gov/pubmed/32821438
http://dx.doi.org/10.1038/s41420-020-00293-z
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author Paldino, Emanuela
D’Angelo, Vincenza
Sancesario, Giuseppe
Fusco, Francesca R.
author_facet Paldino, Emanuela
D’Angelo, Vincenza
Sancesario, Giuseppe
Fusco, Francesca R.
author_sort Paldino, Emanuela
collection PubMed
description Mechanisms of tissue damage in Huntington’s disease involve excitotoxicity, mitochondrial damage, and neuroinflammation, including microglia activation. In the present study, we investigate the role of pyroptosis process in the striatal neurons of the R6/2 mouse model of Huntington’s disease. Transgenic mice were sacrificed at 4 and 13 weeks of age. After sacrifice, histological and immunohistochemical studies were performed. We found that NLRP3 and Caspase-1 were intensely expressed in 13-week-old R6/2 mice. Moreover, NLRP3 expression levels were higher in striatal spiny projection neurons and in parvalbumin interneurons, which are prone to degenerate in HD.
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spelling pubmed-73958072020-08-18 Pyroptotic cell death in the R6/2 mouse model of Huntington’s disease: new insight on the inflammasome Paldino, Emanuela D’Angelo, Vincenza Sancesario, Giuseppe Fusco, Francesca R. Cell Death Discov Article Mechanisms of tissue damage in Huntington’s disease involve excitotoxicity, mitochondrial damage, and neuroinflammation, including microglia activation. In the present study, we investigate the role of pyroptosis process in the striatal neurons of the R6/2 mouse model of Huntington’s disease. Transgenic mice were sacrificed at 4 and 13 weeks of age. After sacrifice, histological and immunohistochemical studies were performed. We found that NLRP3 and Caspase-1 were intensely expressed in 13-week-old R6/2 mice. Moreover, NLRP3 expression levels were higher in striatal spiny projection neurons and in parvalbumin interneurons, which are prone to degenerate in HD. Nature Publishing Group UK 2020-07-31 /pmc/articles/PMC7395807/ /pubmed/32821438 http://dx.doi.org/10.1038/s41420-020-00293-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Paldino, Emanuela
D’Angelo, Vincenza
Sancesario, Giuseppe
Fusco, Francesca R.
Pyroptotic cell death in the R6/2 mouse model of Huntington’s disease: new insight on the inflammasome
title Pyroptotic cell death in the R6/2 mouse model of Huntington’s disease: new insight on the inflammasome
title_full Pyroptotic cell death in the R6/2 mouse model of Huntington’s disease: new insight on the inflammasome
title_fullStr Pyroptotic cell death in the R6/2 mouse model of Huntington’s disease: new insight on the inflammasome
title_full_unstemmed Pyroptotic cell death in the R6/2 mouse model of Huntington’s disease: new insight on the inflammasome
title_short Pyroptotic cell death in the R6/2 mouse model of Huntington’s disease: new insight on the inflammasome
title_sort pyroptotic cell death in the r6/2 mouse model of huntington’s disease: new insight on the inflammasome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395807/
https://www.ncbi.nlm.nih.gov/pubmed/32821438
http://dx.doi.org/10.1038/s41420-020-00293-z
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