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Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity

The targeted nuclease clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has recently emerged as a prominent gene manipulation method. Because of its ease in programming targeted DNA/protein binding through RNA in a vast range of organisms, this...

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Detalles Bibliográficos
Autores principales: Shin, Juhyun, Oh, Jae-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396914/
https://www.ncbi.nlm.nih.gov/pubmed/32580834
http://dx.doi.org/10.5483/BMBRep.2020.53.7.070
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author Shin, Juhyun
Oh, Jae-Wook
author_facet Shin, Juhyun
Oh, Jae-Wook
author_sort Shin, Juhyun
collection PubMed
description The targeted nuclease clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has recently emerged as a prominent gene manipulation method. Because of its ease in programming targeted DNA/protein binding through RNA in a vast range of organisms, this prokaryotic defense system is a versatile tool with many applications in the research field as well as high potential in agricultural and clinical improvements. This review will present a brief history that led to its discovery and adaptation. We also present some of its restrictions, and modifications that have been performed to overcome such restrictions, focusing specifically on the most common CRISPR/Cas9 mediated non-homologous end joint repair.
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spelling pubmed-73969142020-08-10 Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity Shin, Juhyun Oh, Jae-Wook BMB Rep Invited Mini Review The targeted nuclease clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has recently emerged as a prominent gene manipulation method. Because of its ease in programming targeted DNA/protein binding through RNA in a vast range of organisms, this prokaryotic defense system is a versatile tool with many applications in the research field as well as high potential in agricultural and clinical improvements. This review will present a brief history that led to its discovery and adaptation. We also present some of its restrictions, and modifications that have been performed to overcome such restrictions, focusing specifically on the most common CRISPR/Cas9 mediated non-homologous end joint repair. Korean Society for Biochemistry and Molecular Biology 2020-07-31 2020-07-31 /pmc/articles/PMC7396914/ /pubmed/32580834 http://dx.doi.org/10.5483/BMBRep.2020.53.7.070 Text en Copyright © 2020 by the The Korean Society for Biochemistry and Molecular Biology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Invited Mini Review
Shin, Juhyun
Oh, Jae-Wook
Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity
title Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity
title_full Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity
title_fullStr Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity
title_full_unstemmed Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity
title_short Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity
title_sort development of crispr/cas9 system for targeted dna modifications and recent improvements in modification efficiency and specificity
topic Invited Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396914/
https://www.ncbi.nlm.nih.gov/pubmed/32580834
http://dx.doi.org/10.5483/BMBRep.2020.53.7.070
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