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Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity
The targeted nuclease clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has recently emerged as a prominent gene manipulation method. Because of its ease in programming targeted DNA/protein binding through RNA in a vast range of organisms, this...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Biochemistry and Molecular Biology
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396914/ https://www.ncbi.nlm.nih.gov/pubmed/32580834 http://dx.doi.org/10.5483/BMBRep.2020.53.7.070 |
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author | Shin, Juhyun Oh, Jae-Wook |
author_facet | Shin, Juhyun Oh, Jae-Wook |
author_sort | Shin, Juhyun |
collection | PubMed |
description | The targeted nuclease clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has recently emerged as a prominent gene manipulation method. Because of its ease in programming targeted DNA/protein binding through RNA in a vast range of organisms, this prokaryotic defense system is a versatile tool with many applications in the research field as well as high potential in agricultural and clinical improvements. This review will present a brief history that led to its discovery and adaptation. We also present some of its restrictions, and modifications that have been performed to overcome such restrictions, focusing specifically on the most common CRISPR/Cas9 mediated non-homologous end joint repair. |
format | Online Article Text |
id | pubmed-7396914 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Korean Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-73969142020-08-10 Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity Shin, Juhyun Oh, Jae-Wook BMB Rep Invited Mini Review The targeted nuclease clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has recently emerged as a prominent gene manipulation method. Because of its ease in programming targeted DNA/protein binding through RNA in a vast range of organisms, this prokaryotic defense system is a versatile tool with many applications in the research field as well as high potential in agricultural and clinical improvements. This review will present a brief history that led to its discovery and adaptation. We also present some of its restrictions, and modifications that have been performed to overcome such restrictions, focusing specifically on the most common CRISPR/Cas9 mediated non-homologous end joint repair. Korean Society for Biochemistry and Molecular Biology 2020-07-31 2020-07-31 /pmc/articles/PMC7396914/ /pubmed/32580834 http://dx.doi.org/10.5483/BMBRep.2020.53.7.070 Text en Copyright © 2020 by the The Korean Society for Biochemistry and Molecular Biology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Invited Mini Review Shin, Juhyun Oh, Jae-Wook Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity |
title | Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity |
title_full | Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity |
title_fullStr | Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity |
title_full_unstemmed | Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity |
title_short | Development of CRISPR/Cas9 system for targeted DNA modifications and recent improvements in modification efficiency and specificity |
title_sort | development of crispr/cas9 system for targeted dna modifications and recent improvements in modification efficiency and specificity |
topic | Invited Mini Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396914/ https://www.ncbi.nlm.nih.gov/pubmed/32580834 http://dx.doi.org/10.5483/BMBRep.2020.53.7.070 |
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