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Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells

In mammals, conventional B (B2) cells are activated within lymphoid follicles through a close relationship with T follicular helper (Tfh) cells. The interaction between CD40 expressed on B cells and its ligand (CD40L) expressed on Tfh cells is a key signal that regulates the formation of germinal ce...

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Autores principales: Abos, Beatriz, Wang, Tiehui, Secombes, Christopher J., Tafalla, Carolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397517/
https://www.ncbi.nlm.nih.gov/pubmed/32447012
http://dx.doi.org/10.1016/j.dci.2020.103752
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author Abos, Beatriz
Wang, Tiehui
Secombes, Christopher J.
Tafalla, Carolina
author_facet Abos, Beatriz
Wang, Tiehui
Secombes, Christopher J.
Tafalla, Carolina
author_sort Abos, Beatriz
collection PubMed
description In mammals, conventional B (B2) cells are activated within lymphoid follicles through a close relationship with T follicular helper (Tfh) cells. The interaction between CD40 expressed on B cells and its ligand (CD40L) expressed on Tfh cells is a key signal that regulates the formation of germinal centers (GCs), B cell survival, proliferation and differentiation to plasma cells (PCs) or memory cells. Additionally, certain soluble cytokines produced by T cells also strongly condition the outcome of this interaction. Despite the many differences found between fish B cells and mammalian B2 cells, and the lack of conventional GCs, rainbow trout IgM(+) B cells have been shown to be stimulated by CD40L, however, whether cytokines commonly produced by T cells can further modulate this response has never been addressed to date. Thus, in this study, we determined the effects of recombinant rainbow trout adaptive cytokines interleukin 2B (IL-2B), IL-4/13A, IL-4/13B, IL-10 and IL-21 (cytokines known to activate B cells in mammals) on splenic IgM(+) B cells alone or in combination with CD40L. We studied how these cytokines and CD40L cooperated to promote IgM(+) B cell survival, proliferation and IgM secretion. The results obtained provide valuable information for the first time in teleost fish on how different T cell signals cooperate to activate B cells in the absence of GCs.
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spelling pubmed-73975172020-10-01 Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells Abos, Beatriz Wang, Tiehui Secombes, Christopher J. Tafalla, Carolina Dev Comp Immunol Article In mammals, conventional B (B2) cells are activated within lymphoid follicles through a close relationship with T follicular helper (Tfh) cells. The interaction between CD40 expressed on B cells and its ligand (CD40L) expressed on Tfh cells is a key signal that regulates the formation of germinal centers (GCs), B cell survival, proliferation and differentiation to plasma cells (PCs) or memory cells. Additionally, certain soluble cytokines produced by T cells also strongly condition the outcome of this interaction. Despite the many differences found between fish B cells and mammalian B2 cells, and the lack of conventional GCs, rainbow trout IgM(+) B cells have been shown to be stimulated by CD40L, however, whether cytokines commonly produced by T cells can further modulate this response has never been addressed to date. Thus, in this study, we determined the effects of recombinant rainbow trout adaptive cytokines interleukin 2B (IL-2B), IL-4/13A, IL-4/13B, IL-10 and IL-21 (cytokines known to activate B cells in mammals) on splenic IgM(+) B cells alone or in combination with CD40L. We studied how these cytokines and CD40L cooperated to promote IgM(+) B cell survival, proliferation and IgM secretion. The results obtained provide valuable information for the first time in teleost fish on how different T cell signals cooperate to activate B cells in the absence of GCs. Elsevier Science 2020-10 /pmc/articles/PMC7397517/ /pubmed/32447012 http://dx.doi.org/10.1016/j.dci.2020.103752 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Abos, Beatriz
Wang, Tiehui
Secombes, Christopher J.
Tafalla, Carolina
Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells
title Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells
title_full Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells
title_fullStr Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells
title_full_unstemmed Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells
title_short Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells
title_sort distinct modes of action of cd40l and adaptive cytokines il-2, il-4/13, il-10 and il-21 on rainbow trout igm(+) b cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397517/
https://www.ncbi.nlm.nih.gov/pubmed/32447012
http://dx.doi.org/10.1016/j.dci.2020.103752
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