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Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells
In mammals, conventional B (B2) cells are activated within lymphoid follicles through a close relationship with T follicular helper (Tfh) cells. The interaction between CD40 expressed on B cells and its ligand (CD40L) expressed on Tfh cells is a key signal that regulates the formation of germinal ce...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397517/ https://www.ncbi.nlm.nih.gov/pubmed/32447012 http://dx.doi.org/10.1016/j.dci.2020.103752 |
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author | Abos, Beatriz Wang, Tiehui Secombes, Christopher J. Tafalla, Carolina |
author_facet | Abos, Beatriz Wang, Tiehui Secombes, Christopher J. Tafalla, Carolina |
author_sort | Abos, Beatriz |
collection | PubMed |
description | In mammals, conventional B (B2) cells are activated within lymphoid follicles through a close relationship with T follicular helper (Tfh) cells. The interaction between CD40 expressed on B cells and its ligand (CD40L) expressed on Tfh cells is a key signal that regulates the formation of germinal centers (GCs), B cell survival, proliferation and differentiation to plasma cells (PCs) or memory cells. Additionally, certain soluble cytokines produced by T cells also strongly condition the outcome of this interaction. Despite the many differences found between fish B cells and mammalian B2 cells, and the lack of conventional GCs, rainbow trout IgM(+) B cells have been shown to be stimulated by CD40L, however, whether cytokines commonly produced by T cells can further modulate this response has never been addressed to date. Thus, in this study, we determined the effects of recombinant rainbow trout adaptive cytokines interleukin 2B (IL-2B), IL-4/13A, IL-4/13B, IL-10 and IL-21 (cytokines known to activate B cells in mammals) on splenic IgM(+) B cells alone or in combination with CD40L. We studied how these cytokines and CD40L cooperated to promote IgM(+) B cell survival, proliferation and IgM secretion. The results obtained provide valuable information for the first time in teleost fish on how different T cell signals cooperate to activate B cells in the absence of GCs. |
format | Online Article Text |
id | pubmed-7397517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-73975172020-10-01 Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells Abos, Beatriz Wang, Tiehui Secombes, Christopher J. Tafalla, Carolina Dev Comp Immunol Article In mammals, conventional B (B2) cells are activated within lymphoid follicles through a close relationship with T follicular helper (Tfh) cells. The interaction between CD40 expressed on B cells and its ligand (CD40L) expressed on Tfh cells is a key signal that regulates the formation of germinal centers (GCs), B cell survival, proliferation and differentiation to plasma cells (PCs) or memory cells. Additionally, certain soluble cytokines produced by T cells also strongly condition the outcome of this interaction. Despite the many differences found between fish B cells and mammalian B2 cells, and the lack of conventional GCs, rainbow trout IgM(+) B cells have been shown to be stimulated by CD40L, however, whether cytokines commonly produced by T cells can further modulate this response has never been addressed to date. Thus, in this study, we determined the effects of recombinant rainbow trout adaptive cytokines interleukin 2B (IL-2B), IL-4/13A, IL-4/13B, IL-10 and IL-21 (cytokines known to activate B cells in mammals) on splenic IgM(+) B cells alone or in combination with CD40L. We studied how these cytokines and CD40L cooperated to promote IgM(+) B cell survival, proliferation and IgM secretion. The results obtained provide valuable information for the first time in teleost fish on how different T cell signals cooperate to activate B cells in the absence of GCs. Elsevier Science 2020-10 /pmc/articles/PMC7397517/ /pubmed/32447012 http://dx.doi.org/10.1016/j.dci.2020.103752 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Abos, Beatriz Wang, Tiehui Secombes, Christopher J. Tafalla, Carolina Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells |
title | Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells |
title_full | Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells |
title_fullStr | Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells |
title_full_unstemmed | Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells |
title_short | Distinct modes of action of CD40L and adaptive cytokines IL-2, IL-4/13, IL-10 and IL-21 on rainbow trout IgM(+) B cells |
title_sort | distinct modes of action of cd40l and adaptive cytokines il-2, il-4/13, il-10 and il-21 on rainbow trout igm(+) b cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397517/ https://www.ncbi.nlm.nih.gov/pubmed/32447012 http://dx.doi.org/10.1016/j.dci.2020.103752 |
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