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Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread adoption. Here we provide a review of cell-extract methods, with a specific focus on prokaryotic systems. Firstly, we describe the...

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Detalles Bibliográficos
Autores principales: Cole, Stephanie D., Miklos, Aleksandr E., Chiao, Abel C., Sun, Zachary Z., Lux, Matthew W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7398980/
https://www.ncbi.nlm.nih.gov/pubmed/32775710
http://dx.doi.org/10.1016/j.synbio.2020.07.006