Enhancement of extracellular bispecific anti‐MUC1 nanobody expression in E. coli BL21 (DE3) by optimization of temperature and carbon sources through an autoinduction condition

Escherichia coli is one of the most suitable hosts for production of antibodies and antibody fragments. Antibody fragment secretion to the culture medium improves product purity in cell culture and diminishes downstream costs. In this study, E. coli strain BL21 (DE3) harboring gene encoding bispecif...

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Detalles Bibliográficos
Autores principales: Rezaei, Leila, Shojaosadati, Seyed Abbas, Farahmand, Leila, Moradi‐Kalbolandi, Shima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401236/
https://www.ncbi.nlm.nih.gov/pubmed/32774206
http://dx.doi.org/10.1002/elsc.201900158

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401236/
https://www.ncbi.nlm.nih.gov/pubmed/32774206
http://dx.doi.org/10.1002/elsc.201900158

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