Transposase-assisted tagmentation of RNA/DNA hybrid duplexes

Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro. As a proof-of-concept application, we utilized this activity to re...

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Detalles Bibliográficos
Autores principales: Lu, Bo, Dong, Liting, Yi, Danyang, Zhang, Meiling, Zhu, Chenxu, Li, Xiaoyu, Yi, Chengqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Biochemistry and Chemical Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7402673/
https://www.ncbi.nlm.nih.gov/pubmed/32701057
http://dx.doi.org/10.7554/eLife.54919

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7402673/
https://www.ncbi.nlm.nih.gov/pubmed/32701057
http://dx.doi.org/10.7554/eLife.54919

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