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Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR

Quantitative PCR (qPCR) is a widely used method for nucleic acid quantification of various pathogenic microorganisms. For absolute quantification of microbial load by qPCR, it is essential to create a calibration curve from accurately quantified quantification standards, from which the number of pat...

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Detalles Bibliográficos
Autores principales: Beinhauerova, Martina, Babak, Vladimir, Bertasi, Barbara, Boniotti, Maria Beatrice, Kralik, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403525/
https://www.ncbi.nlm.nih.gov/pubmed/32850953
http://dx.doi.org/10.3389/fmolb.2020.00155