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Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR
Quantitative PCR (qPCR) is a widely used method for nucleic acid quantification of various pathogenic microorganisms. For absolute quantification of microbial load by qPCR, it is essential to create a calibration curve from accurately quantified quantification standards, from which the number of pat...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403525/ https://www.ncbi.nlm.nih.gov/pubmed/32850953 http://dx.doi.org/10.3389/fmolb.2020.00155 |