Quantitative analysis of heparan sulfate using isotopically labeled calibrants

Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight (13)C-labeled disaccharide calibrants and one...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Zhangjie, Arnold, Katelyn, Xu, Yongmei, Pagadala, Vijayakanth, Su, Guowei, Myatt, Hannah, Linhardt, Robert J., Liu, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403576/
https://www.ncbi.nlm.nih.gov/pubmed/32753592
http://dx.doi.org/10.1038/s42003-020-01150-6
Descripción
Sumario:Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight (13)C-labeled disaccharide calibrants and one (13)C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.

Ejemplares similares