Quantitative analysis of heparan sulfate using isotopically labeled calibrants

Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight (13)C-labeled disaccharide calibrants and one...

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Autores principales: Wang, Zhangjie, Arnold, Katelyn, Xu, Yongmei, Pagadala, Vijayakanth, Su, Guowei, Myatt, Hannah, Linhardt, Robert J., Liu, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403576/
https://www.ncbi.nlm.nih.gov/pubmed/32753592
http://dx.doi.org/10.1038/s42003-020-01150-6
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author Wang, Zhangjie
Arnold, Katelyn
Xu, Yongmei
Pagadala, Vijayakanth
Su, Guowei
Myatt, Hannah
Linhardt, Robert J.
Liu, Jian
author_facet Wang, Zhangjie
Arnold, Katelyn
Xu, Yongmei
Pagadala, Vijayakanth
Su, Guowei
Myatt, Hannah
Linhardt, Robert J.
Liu, Jian
author_sort Wang, Zhangjie
collection PubMed
description Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight (13)C-labeled disaccharide calibrants and one (13)C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.
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spelling pubmed-74035762020-08-13 Quantitative analysis of heparan sulfate using isotopically labeled calibrants Wang, Zhangjie Arnold, Katelyn Xu, Yongmei Pagadala, Vijayakanth Su, Guowei Myatt, Hannah Linhardt, Robert J. Liu, Jian Commun Biol Article Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight (13)C-labeled disaccharide calibrants and one (13)C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma. Nature Publishing Group UK 2020-08-04 /pmc/articles/PMC7403576/ /pubmed/32753592 http://dx.doi.org/10.1038/s42003-020-01150-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Zhangjie
Arnold, Katelyn
Xu, Yongmei
Pagadala, Vijayakanth
Su, Guowei
Myatt, Hannah
Linhardt, Robert J.
Liu, Jian
Quantitative analysis of heparan sulfate using isotopically labeled calibrants
title Quantitative analysis of heparan sulfate using isotopically labeled calibrants
title_full Quantitative analysis of heparan sulfate using isotopically labeled calibrants
title_fullStr Quantitative analysis of heparan sulfate using isotopically labeled calibrants
title_full_unstemmed Quantitative analysis of heparan sulfate using isotopically labeled calibrants
title_short Quantitative analysis of heparan sulfate using isotopically labeled calibrants
title_sort quantitative analysis of heparan sulfate using isotopically labeled calibrants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403576/
https://www.ncbi.nlm.nih.gov/pubmed/32753592
http://dx.doi.org/10.1038/s42003-020-01150-6
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