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Label-Free Multiple Reaction Monitoring, a Promising Method for Quantification Analyses of Specific Proteins in Bacteria

Bacillus subtilis produces eight industrially important exo-proteases. For the detection of proteases, the activity- and antibody-based assays are normally used. Current activity-based assays require expensive multiplex chemical substrates which allow specificity determination of each enzyme. In thi...

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Detalles Bibliográficos
Autores principales: Toymentseva, Anna A., Koryagina, Anastasia O., Laikov, Alexander V., Sharipova, Margarita R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404251/
https://www.ncbi.nlm.nih.gov/pubmed/32664686
http://dx.doi.org/10.3390/ijms21144924