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Discrimination of CpG Methylation Status and Nucleotide Differences in Tissue Specimen DNA by Oligoribonucleotide Interference-PCR

Oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) is a method in which PCR amplification of a target sequence is inhibited in a sequence-specific manner by the hybridization of an ORN with the target sequence. Previously, we reported that ORNi-PCR could detect nucleotide mutations in DNA purifie...

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Detalles Bibliográficos
Autores principales: Shimizu, Takeshi, Fujita, Toshitsugu, Fukushi, Sakie, Horino, Yuri, Fujii, Hodaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404293/
https://www.ncbi.nlm.nih.gov/pubmed/32698480
http://dx.doi.org/10.3390/ijms21145119