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Cloning antibodies from single cells in pooled sequence libraries by selective PCR
Antibodies function by binding to antigens. Antibodies must be cloned and expressed to determine their binding characteristics, but current methods for high-throughput antibody sequencing yield antibody DNA pooled from many cells and do not readily permit cloning of antibodies from single B cells. W...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406036/ https://www.ncbi.nlm.nih.gov/pubmed/32756607 http://dx.doi.org/10.1371/journal.pone.0236477 |