Improvement of CRISPR/Cas9 system by transfecting Cas9-expressing Plasmodium berghei with linear donor template

Malaria is caused by infection with Plasmodium parasites and is a major public health concern. The CRISPR/Cas9 system is a promising technology, but still has technical problems, such as low efficiency and unexpected recombination. Here, we solved these problems by transfecting Cas9-expressing paras...

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Detalles Bibliográficos
Autores principales: Shinzawa, Naoaki, Nishi, Tsubasa, Hiyoshi, Fumiya, Motooka, Daisuke, Yuda, Masao, Iwanaga, Shiroh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406498/
https://www.ncbi.nlm.nih.gov/pubmed/32759952
http://dx.doi.org/10.1038/s42003-020-01138-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406498/
https://www.ncbi.nlm.nih.gov/pubmed/32759952
http://dx.doi.org/10.1038/s42003-020-01138-2

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