Optimized Protocol for the In Situ Derivatization of Glutathione with N-Ethylmaleimide in Cultured Cells and the Simultaneous Determination of Glutathione/Glutathione Disulfide Ratio by HPLC-UV-QTOF-MS

Glutathione (GSH) and glutathione disulfide (GSSG) are commonly used to assess the oxidative status of a biological system. Various protocols are available for the analysis of GSH and GSSG in biomedical specimens. In this study, we present an optimized protocol for the in situ derivatization of GSH...

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Detalles Bibliográficos
Autores principales: Sun, Xueni, Berger, Raffaela S., Heinrich, Paul, Marchiq, Ibtissam, Pouyssegur, Jacques, Renner, Kathrin, Oefner, Peter J., Dettmer, Katja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407321/
https://www.ncbi.nlm.nih.gov/pubmed/32709039
http://dx.doi.org/10.3390/metabo10070292

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407321/
https://www.ncbi.nlm.nih.gov/pubmed/32709039
http://dx.doi.org/10.3390/metabo10070292

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