Cargando…

Identification of a Clinical Cutoff Value for Multiplex KRAS(G12/G13) Mutation Detection in Colorectal Adenocarcinoma Patients Using Digital Droplet PCR, and Comparison with Sanger Sequencing and PNA Clamping Assay

KRAS (Kirsten rat sarcoma 2 viral oncogene homolog) is a major predictive marker for anti-epidermal growth factor receptor treatment, and determination of KRAS mutational status is crucial for successful management of colorectal adenocarcinoma. More standardized and accurate methods for testing KRAS...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lee, Kyung Ha, Lee, Tae Hee, Choi, Min Kyung, Kwon, In Sun, Bae, Go Eun, Yeo, Min-Kyung
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409004/ https://www.ncbi.nlm.nih.gov/pubmed/32708359 http://dx.doi.org/10.3390/jcm9072283

Ejemplares similares

Serum-Based KRAS(G12/G13) Mutation Detection Using Droplet Digital PCR: Clinical Implications and Limitations in Colorectal Adenocarcinoma With Tumor Heterogeneity
por: Kim, Ju Seok, et al.
Publicado: (2021)

Detection of EGFR mutations in circulating free DNA by PNA-mediated PCR clamping
por: Kim, Hye-Ryoun, et al.
Publicado: (2013)

Detection of KRAS G12D in colorectal cancer stool by droplet digital PCR
por: Olmedillas-López, Susana, et al.
Publicado: (2017)

EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method
por: Watanabe, Kana, et al.
Publicado: (2016)

KRAS Mutation Test in Korean Patients with Colorectal Carcinomas: A Methodological Comparison between Sanger Sequencing and a Real-Time PCR-Based Assay
por: Lee, Sung Hak, et al.
Publicado: (2017)

Screening PCR Versus Sanger Sequencing: Detection of CALR Mutations in Patients With Thrombocytosis
por: Jeong, Ji Hun, et al.
Publicado: (2016)

Detection of circulating KRAS mutant DNA in extracellular vesicles using droplet digital PCR in patients with colon cancer
por: Choi, Jeesoo, et al.
Publicado: (2022)

Characteristics and prognostic analysis of patients with detected KRAS mutations in resected lung adenocarcinomas by peptide nucleic acid-locked nucleic acid polymerase chain reaction (PNA-LNA PCR) clamp method
por: Hashimoto, Tatsuya, et al.
Publicado: (2023)

Validation of a Multiplex Assay for Measuring Specific IgG4
por: Kim, Sung-Ryeol, et al.
Publicado: (2020)

PNA clamping-assisted fluorescence melting curve analysis for detecting EGFR and KRAS mutations in the circulating tumor DNA of patients with advanced non-small cell lung cancer
por: Han, Ji-Youn, et al.
Publicado: (2016)

Development of a Detection System for ESR1 Mutations in Circulating Tumour DNA Using PNA-LNA-Mediated PCR Clamping
por: Kojima, Yuki, et al.
Publicado: (2023)

Erratum to: PNA clamping-assisted fluorescence melting curve analysis for detecting EGFR and KRAS mutations in the circulating tumor DNA of patients with advanced non-small cell lung cancer
por: Han, Ji-Youn, et al.
Publicado: (2016)

KRAS G12V Mutation Detection by Droplet Digital PCR in Circulating Cell-Free DNA of Colorectal Cancer Patients
por: Olmedillas López, Susana, et al.
Publicado: (2016)

Detection of Ki-ras mutations in tissue and plasma samples of patients with pancreatic cancer using PNA-mediated PCR clamping and hybridisation probes
por: Däbritz, J, et al.
Publicado: (2005)

Detection of K-Ras mutations in tumour samples of patients with non-small cell lung cancer using PNA-mediated PCR clamping
por: Beau-Faller, M, et al.
Publicado: (2009)

Detection of SF3B1 p.Lys700Glu Mutation by PNA-PCR Clamping in Myelodysplastic Syndromes and Myeloproliferative Neoplasms
por: Petiti, Jessica, et al.
Publicado: (2022)

Efficient Genotyping of KRAS Mutant Non-Small Cell Lung Cancer Using a Multiplexed Droplet Digital PCR Approach
por: Pender, Alexandra, et al.
Publicado: (2015)

Diagnosis of Fanconi Anemia: Mutation Analysis by Multiplex Ligation-Dependent Probe Amplification and PCR-Based Sanger Sequencing
por: Gille, Johan J. P., et al.
Publicado: (2012)

Identification of Duplication Genotypes of the Feathering Rate Gene in Chicken by a Multiplex PCR Following Electrophoresis and/or Sanger Sequencing
por: Shen, Qingmiao, et al.
Publicado: (2023)

DISSECT Method Using PNA-LNA Clamp Improves Detection of EGFR T790m Mutation
por: Guha, Minakshi, et al.
Publicado: (2013)

PNA Clamping in Nucleic Acid Amplification Protocols to Detect Single Nucleotide Mutations Related to Cancer
por: Fouz, Munira F., et al.
Publicado: (2020)

Development, evaluation of the PNA RT-LAMP assay for rapid molecular detection of SARS-CoV-2
por: Bat-Ochir, Chinbayar, et al.
Publicado: (2021)

Detection of KRAS G12/G13 Mutations in Cell Free-DNA by Droplet Digital PCR, Offers Prognostic Information for Patients with Advanced Non-Small Cell Lung Cancer
por: Michaelidou, Kleita, et al.
Publicado: (2020)

Comparison of droplet digital PCR and direct Sanger sequencing for the detection of the BRAF (V600E) mutation in papillary thyroid carcinoma
por: Wang, Zhuo, et al.
Publicado: (2019)

Reverse Transcription-PCR-based Sanger Sequencing-confirmed Exon-skipping Effect of a Novel GEN1 Intronic Variant (c.1408+4A>G)
por: Kim, Jiyeon, et al.
Publicado: (2024)

Standardization of Aspergillus IgG diagnostic cutoff in Nigerians
por: Oladele, Rita O., et al.
Publicado: (2021)

Non-Small-Cell Lung Cancer-Sensitive Detection of the p.Thr790Met EGFR Alteration by Preamplification before PNA-Mediated PCR Clamping and Pyrosequencing
por: Billaud, Amandine, et al.
Publicado: (2020)

Publisher Correction: Development, evaluation of the PNA RT-LAMP assay for rapid molecular detection of SARS-CoV-2
por: Bat-Ochir, Chinbayar, et al.
Publicado: (2021)

Detecting KRAS mutations in peripheral blood of colorectal cancer patients by peptide nucleic acid clamp PCR
por: MIYANO, SHOZO, et al.
Publicado: (2012)

Development of a multiplex real-time PCR assay for the simultaneous detection of four bacterial pathogens causing pneumonia
por: Lim, Ho Jae, et al.
Publicado: (2021)

Performance Evaluation of the QXDx BCR-ABL %IS Droplet Digital PCR Assay
por: Chung, Hee-Jung, et al.
Publicado: (2020)

Base pair opening kinetics study of the aegPNA:DNA hydrid duplex containing a site-specific GNA-like chiral PNA monomer
por: Seo, Yeo-Jin, et al.
Publicado: (2011)

Jumping the green wall: The use of PNA‐DNA clamps to enhance microbiome sampling depth in wildlife microbiome research
por: Víquez‐R, Luis, et al.
Publicado: (2020)

Development of a Multiplex Droplet Digital PCR Assay for the Detection of Babesia, Bartonella, and Borrelia Species
por: Maggi, Ricardo, et al.
Publicado: (2021)

SensiScreen(®) KRAS exon 2-sensitive simplex and multiplex real-time PCR-based assays for detection of KRAS exon 2 mutations
por: Riva, Alice, et al.
Publicado: (2017)

HIV reservoir quantification by five-target multiplex droplet digital PCR
por: Levy, Claire N., et al.
Publicado: (2021)

Multiplex detection of “Candidatus Liberibacter asiaticus” and Spiroplasma citri by qPCR and droplet digital PCR
por: Maheshwari, Yogita, et al.
Publicado: (2021)

Increasing the Efficiency of Canola and Soybean GMO Detection and Quantification Using Multiplex Droplet Digital PCR
por: Demeke, Tigst, et al.
Publicado: (2022)

Effectiveness of blocking primers and a peptide nucleic acid (PNA) clamp for 18S metabarcoding dietary analysis of herbivorous fish
por: Homma, Chiho, et al.
Publicado: (2022)

A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir
por: Levy, Claire N., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_bvjekalout4omaavh2jhm3h694