Hydrodynamics-Based Transplacental Delivery as a Useful Noninvasive Tool for Manipulating Fetal Genome

We previously demonstrated that the injection of pregnant wild-type female mice (carrying enhanced green fluorescent protein (EGFP)-expressing transgenic fetuses) at embryonic day (E) 12.5 with an all-in-one plasmid conferring the expression of both Cas9 and guide RNA (targeted to the EGFP cDNA) com...

Descripción completa

Detalles Bibliográficos
Autores principales: Nakamura, Shingo, Ando, Naoko, Watanabe, Satoshi, Akasaka, Eri, Ishihara, Masayuki, Sato, Masahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409276/
https://www.ncbi.nlm.nih.gov/pubmed/32708213
http://dx.doi.org/10.3390/cells9071744

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409276/
https://www.ncbi.nlm.nih.gov/pubmed/32708213
http://dx.doi.org/10.3390/cells9071744

Ejemplares similares