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There is no magic bullet: the importance of testing reference gene stability in RT-qPCR experiments across multiple closely related species

Reverse Transcriptase quantitative Polymerase Chain Reaction (RT-qPCR) is the current gold standard tool for the study of gene expression. This technique is highly dependent on the validation of reference genes, which exhibit stable expression levels among experimental conditions. Often, reference g...

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Detalles Bibliográficos
Autores principales: Foquet, Bert, Song, Hojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409783/
https://www.ncbi.nlm.nih.gov/pubmed/32832268
http://dx.doi.org/10.7717/peerj.9618