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A versatile bulk electrotransfection protocol for murine embryonic fibroblasts and iPS cells
Although electroporation has been widely accepted as the main gene transfer tool, there is still considerable scope to improve the electroporation efficiency of exogenous DNAs into primary cells. Here, we developed a square-wave pulsing protocol using OptiMEM-GlutaMAX for highly efficient transfecti...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7414887/ https://www.ncbi.nlm.nih.gov/pubmed/32770110 http://dx.doi.org/10.1038/s41598-020-70258-w |