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Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction

The genetic identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on viral RNA extraction prior to RT-qPCR assay. However, recent studies have supported the elimination of the extraction step. This study was performed to assess the necessity for the RNA extraction,...

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Detalles Bibliográficos
Autores principales: Israeli, Ofir, Beth-Din, Adi, Paran, Nir, Stein, Dana, Lazar, Shirley, Weiss, Shay, Milrot, Elad, Atiya-Nasagi, Yafit, Yitzhaki, Shmuel, Laskar, Orly, Schuster, Ofir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416699/
https://www.ncbi.nlm.nih.gov/pubmed/32791207
http://dx.doi.org/10.1016/j.ijid.2020.08.015