Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction

The genetic identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on viral RNA extraction prior to RT-qPCR assay. However, recent studies have supported the elimination of the extraction step. This study was performed to assess the necessity for the RNA extraction,...

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Autores principales: Israeli, Ofir, Beth-Din, Adi, Paran, Nir, Stein, Dana, Lazar, Shirley, Weiss, Shay, Milrot, Elad, Atiya-Nasagi, Yafit, Yitzhaki, Shmuel, Laskar, Orly, Schuster, Ofir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416699/
https://www.ncbi.nlm.nih.gov/pubmed/32791207
http://dx.doi.org/10.1016/j.ijid.2020.08.015
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author Israeli, Ofir
Beth-Din, Adi
Paran, Nir
Stein, Dana
Lazar, Shirley
Weiss, Shay
Milrot, Elad
Atiya-Nasagi, Yafit
Yitzhaki, Shmuel
Laskar, Orly
Schuster, Ofir
author_facet Israeli, Ofir
Beth-Din, Adi
Paran, Nir
Stein, Dana
Lazar, Shirley
Weiss, Shay
Milrot, Elad
Atiya-Nasagi, Yafit
Yitzhaki, Shmuel
Laskar, Orly
Schuster, Ofir
author_sort Israeli, Ofir
collection PubMed
description The genetic identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on viral RNA extraction prior to RT-qPCR assay. However, recent studies have supported the elimination of the extraction step. This study was performed to assess the necessity for the RNA extraction, by comparing the efficacy of RT-qPCR in several direct approaches versus the gold standard RNA extraction, in the detection of SARS-CoV-2 in laboratory samples, as well as in clinical oro-nasopharyngeal SARS-CoV-2 swabs. The findings showed an advantage for the extraction procedure; however a direct no-buffer approach might be an alternative, since it identified more than 60% of positive clinical specimens.
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spelling pubmed-74166992020-08-10 Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction Israeli, Ofir Beth-Din, Adi Paran, Nir Stein, Dana Lazar, Shirley Weiss, Shay Milrot, Elad Atiya-Nasagi, Yafit Yitzhaki, Shmuel Laskar, Orly Schuster, Ofir Int J Infect Dis Article The genetic identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on viral RNA extraction prior to RT-qPCR assay. However, recent studies have supported the elimination of the extraction step. This study was performed to assess the necessity for the RNA extraction, by comparing the efficacy of RT-qPCR in several direct approaches versus the gold standard RNA extraction, in the detection of SARS-CoV-2 in laboratory samples, as well as in clinical oro-nasopharyngeal SARS-CoV-2 swabs. The findings showed an advantage for the extraction procedure; however a direct no-buffer approach might be an alternative, since it identified more than 60% of positive clinical specimens. The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2020-10 2020-08-10 /pmc/articles/PMC7416699/ /pubmed/32791207 http://dx.doi.org/10.1016/j.ijid.2020.08.015 Text en © 2020 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Israeli, Ofir
Beth-Din, Adi
Paran, Nir
Stein, Dana
Lazar, Shirley
Weiss, Shay
Milrot, Elad
Atiya-Nasagi, Yafit
Yitzhaki, Shmuel
Laskar, Orly
Schuster, Ofir
Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
title Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
title_full Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
title_fullStr Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
title_full_unstemmed Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
title_short Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
title_sort evaluating the efficacy of rt-qpcr sars-cov-2 direct approaches in comparison to rna extraction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416699/
https://www.ncbi.nlm.nih.gov/pubmed/32791207
http://dx.doi.org/10.1016/j.ijid.2020.08.015
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