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Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells

α/β-tubulin heterodimers, which can harbor diverse isotypes and post-translational modifications, polymerize into microtubules that are fundamental to many cellular processes. Due to long-standing challenges in generating recombinant tubulin, however, it has been difficult to examine the properties...

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Detalles Bibliográficos
Autores principales: Ti, Shih-Chieh, Wieczorek, Michal, Kapoor, Tarun M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416841/
https://www.ncbi.nlm.nih.gov/pubmed/32783031
http://dx.doi.org/10.1016/j.xpro.2019.100011