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Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells
α/β-tubulin heterodimers, which can harbor diverse isotypes and post-translational modifications, polymerize into microtubules that are fundamental to many cellular processes. Due to long-standing challenges in generating recombinant tubulin, however, it has been difficult to examine the properties...
Autores principales: | Ti, Shih-Chieh, Wieczorek, Michal, Kapoor, Tarun M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416841/ https://www.ncbi.nlm.nih.gov/pubmed/32783031 http://dx.doi.org/10.1016/j.xpro.2019.100011 |
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