An Efficient Nitroblue Tetrazolium Staining and Bright-Field Microscopy Based Method for Detecting and Quantifying Intracellular Reactive Oxygen Species in Oocytes, Cumulus Cells and Embryos

Assessment of intracellular reactive oxygen species (ROS) is important for evaluating the developmental ability of cumulus-oocyte complexes (COC) and embryos. Although, fluorescence-based 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) staining method is used widely for detecting intracellular...

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Detalles Bibliográficos
Autores principales: Javvaji, Pradeep K., Dhali, Arindam, Francis, Joseph R., Kolte, Atul P., Mech, Anjumoni, Roy, Sudhir C., Mishra, Ashish, Bhatta, Raghavendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417451/
https://www.ncbi.nlm.nih.gov/pubmed/32850864
http://dx.doi.org/10.3389/fcell.2020.00764

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417451/
https://www.ncbi.nlm.nih.gov/pubmed/32850864
http://dx.doi.org/10.3389/fcell.2020.00764

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