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Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages

Myeloperoxidase (MPO) is a vital component of the innate immune system, which produces the potent oxidant hypochlorous acid (HOCl) to kill invading pathogens. However, an overproduction of HOCl during chronic inflammatory conditions causes damage to host cells, which promotes disease, including athe...

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Autores principales: Guo, Chaorui, Davies, Michael J., Hawkins, Clare L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417949/
https://www.ncbi.nlm.nih.gov/pubmed/32781424
http://dx.doi.org/10.1016/j.redox.2020.101666
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author Guo, Chaorui
Davies, Michael J.
Hawkins, Clare L.
author_facet Guo, Chaorui
Davies, Michael J.
Hawkins, Clare L.
author_sort Guo, Chaorui
collection PubMed
description Myeloperoxidase (MPO) is a vital component of the innate immune system, which produces the potent oxidant hypochlorous acid (HOCl) to kill invading pathogens. However, an overproduction of HOCl during chronic inflammatory conditions causes damage to host cells, which promotes disease, including atherosclerosis. As such, there is increasing interest in the use of thiocyanate (SCN(−)) therapeutically to decrease inflammatory disease, as SCN(−) is the favoured substrate for MPO, and a potent competitive inhibitor of HOCl formation. Use of SCN(−) by MPO forms hypothiocyanous acid (HOSCN), which can be less damaging to mammalian cells. In this study, we examined the ability of SCN(−) to modulate damage to macrophages induced by HOCl, which is relevant to lesion formation in atherosclerosis. Addition of SCN(−) prevented HOCl-mediated cell death, altered the extent and nature of thiol oxidation and the phosphorylation of mitogen activated protein kinases. These changes were dependent on the concentration of SCN(−) and were observed in some cases, at a sub-stoichiometric ratio of SCN(−): HOCl. Co-treatment with SCN(−) also modulated HOCl-induced perturbations in the expression of various antioxidant and inflammatory genes. In general, the data reflect the conversion of HOCl to HOSCN, which can induce reversible modifications that are repairable by cells. However, our data also highlight the ability of HOSCN to increase pro-inflammatory gene expression and cytokine/chemokine release, which may be relevant to the use of SCN(−) therapeutically in atherosclerosis. Overall, this study provides further insight into the cellular pathways by which SCN(−) could exert protective effects on supplementation to decrease the development of chronic inflammatory diseases, such as atherosclerosis.
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spelling pubmed-74179492020-08-14 Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages Guo, Chaorui Davies, Michael J. Hawkins, Clare L. Redox Biol Research Paper Myeloperoxidase (MPO) is a vital component of the innate immune system, which produces the potent oxidant hypochlorous acid (HOCl) to kill invading pathogens. However, an overproduction of HOCl during chronic inflammatory conditions causes damage to host cells, which promotes disease, including atherosclerosis. As such, there is increasing interest in the use of thiocyanate (SCN(−)) therapeutically to decrease inflammatory disease, as SCN(−) is the favoured substrate for MPO, and a potent competitive inhibitor of HOCl formation. Use of SCN(−) by MPO forms hypothiocyanous acid (HOSCN), which can be less damaging to mammalian cells. In this study, we examined the ability of SCN(−) to modulate damage to macrophages induced by HOCl, which is relevant to lesion formation in atherosclerosis. Addition of SCN(−) prevented HOCl-mediated cell death, altered the extent and nature of thiol oxidation and the phosphorylation of mitogen activated protein kinases. These changes were dependent on the concentration of SCN(−) and were observed in some cases, at a sub-stoichiometric ratio of SCN(−): HOCl. Co-treatment with SCN(−) also modulated HOCl-induced perturbations in the expression of various antioxidant and inflammatory genes. In general, the data reflect the conversion of HOCl to HOSCN, which can induce reversible modifications that are repairable by cells. However, our data also highlight the ability of HOSCN to increase pro-inflammatory gene expression and cytokine/chemokine release, which may be relevant to the use of SCN(−) therapeutically in atherosclerosis. Overall, this study provides further insight into the cellular pathways by which SCN(−) could exert protective effects on supplementation to decrease the development of chronic inflammatory diseases, such as atherosclerosis. Elsevier 2020-07-31 /pmc/articles/PMC7417949/ /pubmed/32781424 http://dx.doi.org/10.1016/j.redox.2020.101666 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Guo, Chaorui
Davies, Michael J.
Hawkins, Clare L.
Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages
title Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages
title_full Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages
title_fullStr Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages
title_full_unstemmed Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages
title_short Role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages
title_sort role of thiocyanate in the modulation of myeloperoxidase-derived oxidant induced damage to macrophages
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417949/
https://www.ncbi.nlm.nih.gov/pubmed/32781424
http://dx.doi.org/10.1016/j.redox.2020.101666
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