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Use of a simplified sample processing step without RNA extraction for direct SARS-CoV-2 RT-PCR detection

The severe acute respiratory syndrome coronavirus (SARS-CoV-2) pandemic has resulted in significant shortages of RT-PCR testing supplies including RNA extraction kits. The goal of our study was to determine if a simplified heat-RNA release method would provide comparable detection of SARS-CoV-2 with...

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Detalles Bibliográficos
Autores principales: Barza, Ruby, Patel, Parul, Sabatini, Linda, Singh, Kamaljit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7418644/
https://www.ncbi.nlm.nih.gov/pubmed/32898817
http://dx.doi.org/10.1016/j.jcv.2020.104587