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Perfusion culture maintained with an air-liquid interface to stimulate epithelial cell organization in renal organoids in vitro
BACKGROUND: Organoids derived from induced pluripotent stem (iPS) or embryonic stem (ES) cells have been evaluated as in vitro models of development and disease. However, maintaining these cells under long-term static culture conditions is difficult because of nutrition shortages and waste accumulat...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7422605/ https://www.ncbi.nlm.nih.gov/pubmed/32903332 http://dx.doi.org/10.1186/s42490-019-0017-9 |