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Perfusion culture maintained with an air-liquid interface to stimulate epithelial cell organization in renal organoids in vitro

BACKGROUND: Organoids derived from induced pluripotent stem (iPS) or embryonic stem (ES) cells have been evaluated as in vitro models of development and disease. However, maintaining these cells under long-term static culture conditions is difficult because of nutrition shortages and waste accumulat...

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Detalles Bibliográficos
Autores principales: Sekiya, Sachiko, Kikuchi, Tetsutaro, Shimizu, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7422605/
https://www.ncbi.nlm.nih.gov/pubmed/32903332
http://dx.doi.org/10.1186/s42490-019-0017-9