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One-Day TALEN Assembly Protocol and a Dual-Tagging System for Genome Editing

[Image: see text] This study developed a new rapid transcription activator-like effector nuclease (TALEN) preparation protocol by thoroughly redesigning the widely used Golden Gate TALEN and TAL Effector Kit 2.0. The new protocol can be used to prepare any custom 18-bp binding TALENs in just one day...

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Detalles Bibliográficos
Autores principales: Zhang, Shuyan, Wang, Jun, Wang, Jinke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7424704/
https://www.ncbi.nlm.nih.gov/pubmed/32803065
http://dx.doi.org/10.1021/acsomega.0c02396