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Establishing a density-based method to separate proliferating and senescent cells from bone marrow stromal cells

To assist in the study of cellular aging, we established a new method of enriching physiologically aged bone marrow stromal cells (BMSCs) in animals of any age using a Percoll density gradient centrifugation technique. BMSCs from mice 2 months of age were isolated, and their cellular age determined...

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Detalles Bibliográficos
Autores principales: Xu, Fei, Zhang, Qiang, Wang, Haitao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425507/
https://www.ncbi.nlm.nih.gov/pubmed/32710730
http://dx.doi.org/10.18632/aging.103569