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A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli

Taste receptor cells use multiple signaling pathways to detect chemicals in potential food items. These cells are functionally grouped into different types: Type I cells act as support cells and have glial-like properties; Type II cells detect bitter, sweet, and umami taste stimuli; and Type III cel...

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Autores principales: Dutta Banik, Debarghya, Benfey, Eric D., Martin, Laura E., Kay, Kristen E., Loney, Gregory C., Nelson, Amy R., Ahart, Zachary C., Kemp, Barrett T., Kemp, Bailey R., Torregrossa, Ann-Marie, Medler, Kathryn F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425866/
https://www.ncbi.nlm.nih.gov/pubmed/32790785
http://dx.doi.org/10.1371/journal.pgen.1008925
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author Dutta Banik, Debarghya
Benfey, Eric D.
Martin, Laura E.
Kay, Kristen E.
Loney, Gregory C.
Nelson, Amy R.
Ahart, Zachary C.
Kemp, Barrett T.
Kemp, Bailey R.
Torregrossa, Ann-Marie
Medler, Kathryn F.
author_facet Dutta Banik, Debarghya
Benfey, Eric D.
Martin, Laura E.
Kay, Kristen E.
Loney, Gregory C.
Nelson, Amy R.
Ahart, Zachary C.
Kemp, Barrett T.
Kemp, Bailey R.
Torregrossa, Ann-Marie
Medler, Kathryn F.
author_sort Dutta Banik, Debarghya
collection PubMed
description Taste receptor cells use multiple signaling pathways to detect chemicals in potential food items. These cells are functionally grouped into different types: Type I cells act as support cells and have glial-like properties; Type II cells detect bitter, sweet, and umami taste stimuli; and Type III cells detect sour and salty stimuli. We have identified a new population of taste cells that are broadly tuned to multiple taste stimuli including bitter, sweet, sour, and umami. The goal of this study was to characterize these broadly responsive (BR) taste cells. We used an IP(3)R3-KO mouse (does not release calcium (Ca(2+)) from internal stores in Type II cells when stimulated with bitter, sweet, or umami stimuli) to characterize the BR cells without any potentially confounding input from Type II cells. Using live cell Ca(2+) imaging in isolated taste cells from the IP(3)R3-KO mouse, we found that BR cells are a subset of Type III cells that respond to sour stimuli but also use a PLCβ signaling pathway to respond to bitter, sweet, and umami stimuli. Unlike Type II cells, individual BR cells are broadly tuned and respond to multiple stimuli across different taste modalities. Live cell imaging in a PLCβ3-KO mouse confirmed that BR cells use this signaling pathway to respond to bitter, sweet, and umami stimuli. Short term behavioral assays revealed that BR cells make significant contributions to taste driven behaviors and found that loss of either PLCβ3 in BR cells or IP(3)R3 in Type II cells caused similar behavioral deficits to bitter, sweet, and umami stimuli. Analysis of c-Fos activity in the nucleus of the solitary tract (NTS) also demonstrated that functional Type II and BR cells are required for normal stimulus induced expression.
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spelling pubmed-74258662020-08-20 A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli Dutta Banik, Debarghya Benfey, Eric D. Martin, Laura E. Kay, Kristen E. Loney, Gregory C. Nelson, Amy R. Ahart, Zachary C. Kemp, Barrett T. Kemp, Bailey R. Torregrossa, Ann-Marie Medler, Kathryn F. PLoS Genet Research Article Taste receptor cells use multiple signaling pathways to detect chemicals in potential food items. These cells are functionally grouped into different types: Type I cells act as support cells and have glial-like properties; Type II cells detect bitter, sweet, and umami taste stimuli; and Type III cells detect sour and salty stimuli. We have identified a new population of taste cells that are broadly tuned to multiple taste stimuli including bitter, sweet, sour, and umami. The goal of this study was to characterize these broadly responsive (BR) taste cells. We used an IP(3)R3-KO mouse (does not release calcium (Ca(2+)) from internal stores in Type II cells when stimulated with bitter, sweet, or umami stimuli) to characterize the BR cells without any potentially confounding input from Type II cells. Using live cell Ca(2+) imaging in isolated taste cells from the IP(3)R3-KO mouse, we found that BR cells are a subset of Type III cells that respond to sour stimuli but also use a PLCβ signaling pathway to respond to bitter, sweet, and umami stimuli. Unlike Type II cells, individual BR cells are broadly tuned and respond to multiple stimuli across different taste modalities. Live cell imaging in a PLCβ3-KO mouse confirmed that BR cells use this signaling pathway to respond to bitter, sweet, and umami stimuli. Short term behavioral assays revealed that BR cells make significant contributions to taste driven behaviors and found that loss of either PLCβ3 in BR cells or IP(3)R3 in Type II cells caused similar behavioral deficits to bitter, sweet, and umami stimuli. Analysis of c-Fos activity in the nucleus of the solitary tract (NTS) also demonstrated that functional Type II and BR cells are required for normal stimulus induced expression. Public Library of Science 2020-08-13 /pmc/articles/PMC7425866/ /pubmed/32790785 http://dx.doi.org/10.1371/journal.pgen.1008925 Text en © 2020 Dutta Banik et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dutta Banik, Debarghya
Benfey, Eric D.
Martin, Laura E.
Kay, Kristen E.
Loney, Gregory C.
Nelson, Amy R.
Ahart, Zachary C.
Kemp, Barrett T.
Kemp, Bailey R.
Torregrossa, Ann-Marie
Medler, Kathryn F.
A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli
title A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli
title_full A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli
title_fullStr A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli
title_full_unstemmed A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli
title_short A subset of broadly responsive Type III taste cells contribute to the detection of bitter, sweet and umami stimuli
title_sort subset of broadly responsive type iii taste cells contribute to the detection of bitter, sweet and umami stimuli
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425866/
https://www.ncbi.nlm.nih.gov/pubmed/32790785
http://dx.doi.org/10.1371/journal.pgen.1008925
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