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Switching behaviour of dSTORM dyes in glycerol-containing buffer

To suppress optical aberrations caused by refractive index mismatch, we employ glycerol-immersion objectives in conjunction with fused silica cover glasses and imaging buffers with a high glycerol content. Here we demonstrate that the addition of glycerol to the buffer does not degrade the switching...

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Detalles Bibliográficos
Autores principales: Goossen-Schmidt, Nora C., Schnieder, Marco, Hüve, Jana, Klingauf, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426933/
https://www.ncbi.nlm.nih.gov/pubmed/32792515
http://dx.doi.org/10.1038/s41598-020-70335-0