Switching behaviour of dSTORM dyes in glycerol-containing buffer

To suppress optical aberrations caused by refractive index mismatch, we employ glycerol-immersion objectives in conjunction with fused silica cover glasses and imaging buffers with a high glycerol content. Here we demonstrate that the addition of glycerol to the buffer does not degrade the switching...

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Autores principales: Goossen-Schmidt, Nora C., Schnieder, Marco, Hüve, Jana, Klingauf, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426933/
https://www.ncbi.nlm.nih.gov/pubmed/32792515
http://dx.doi.org/10.1038/s41598-020-70335-0
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author Goossen-Schmidt, Nora C.
Schnieder, Marco
Hüve, Jana
Klingauf, Jürgen
author_facet Goossen-Schmidt, Nora C.
Schnieder, Marco
Hüve, Jana
Klingauf, Jürgen
author_sort Goossen-Schmidt, Nora C.
collection PubMed
description To suppress optical aberrations caused by refractive index mismatch, we employ glycerol-immersion objectives in conjunction with fused silica cover glasses and imaging buffers with a high glycerol content. Here we demonstrate that the addition of glycerol to the buffer does not degrade the switching behaviour of the dyes Alexa Fluor 647 and Alexa Fluor 568 in dSTORM measurements, which shows that this approach is suitable for dSTORM. Additionally, we report evidence that sealed sample geometries as used in our experiments reduce photobleaching due to the lower influx of oxygen into the imaging buffer.
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spelling pubmed-74269332020-08-14 Switching behaviour of dSTORM dyes in glycerol-containing buffer Goossen-Schmidt, Nora C. Schnieder, Marco Hüve, Jana Klingauf, Jürgen Sci Rep Article To suppress optical aberrations caused by refractive index mismatch, we employ glycerol-immersion objectives in conjunction with fused silica cover glasses and imaging buffers with a high glycerol content. Here we demonstrate that the addition of glycerol to the buffer does not degrade the switching behaviour of the dyes Alexa Fluor 647 and Alexa Fluor 568 in dSTORM measurements, which shows that this approach is suitable for dSTORM. Additionally, we report evidence that sealed sample geometries as used in our experiments reduce photobleaching due to the lower influx of oxygen into the imaging buffer. Nature Publishing Group UK 2020-08-13 /pmc/articles/PMC7426933/ /pubmed/32792515 http://dx.doi.org/10.1038/s41598-020-70335-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Goossen-Schmidt, Nora C.
Schnieder, Marco
Hüve, Jana
Klingauf, Jürgen
Switching behaviour of dSTORM dyes in glycerol-containing buffer
title Switching behaviour of dSTORM dyes in glycerol-containing buffer
title_full Switching behaviour of dSTORM dyes in glycerol-containing buffer
title_fullStr Switching behaviour of dSTORM dyes in glycerol-containing buffer
title_full_unstemmed Switching behaviour of dSTORM dyes in glycerol-containing buffer
title_short Switching behaviour of dSTORM dyes in glycerol-containing buffer
title_sort switching behaviour of dstorm dyes in glycerol-containing buffer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426933/
https://www.ncbi.nlm.nih.gov/pubmed/32792515
http://dx.doi.org/10.1038/s41598-020-70335-0
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