Detection of Cryptosporidium parvum in a Red-Eared Slider Turtle (Trachemys scripta elegans), a Noted Invasive Alien Species, Captured in a Rural Aquatic Ecosystem in Eastern Poland
PURPOSE: Little is known about cryptosporidiosis in turtles of invasive alien species (IAS) inhabiting European bodies of fresh water. In this article, we report an occurrence of Cryptosporidium parvum in a red-eared slider turtle (Trachemys scripta elegans) captured in a rural aquatic ecosystem in...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer International Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427710/ https://www.ncbi.nlm.nih.gov/pubmed/32141020 http://dx.doi.org/10.2478/s11686-020-00180-8 |
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author | Rzeżutka, Artur Kaupke, Agnieszka Gorzkowski, Bartłomiej |
author_facet | Rzeżutka, Artur Kaupke, Agnieszka Gorzkowski, Bartłomiej |
author_sort | Rzeżutka, Artur |
collection | PubMed |
description | PURPOSE: Little is known about cryptosporidiosis in turtles of invasive alien species (IAS) inhabiting European bodies of fresh water. In this article, we report an occurrence of Cryptosporidium parvum in a red-eared slider turtle (Trachemys scripta elegans) captured in a rural aquatic ecosystem in eastern Poland. METHODS: A pair of samples consisting of feces and scrapings of intestinal mucosa (taken during necropsy) were collected from 104 animals representing the four IAS turtle species red-eared slider, yellow-bellied slider, false map and Cumberland slider. The animals were trapped in running and standing freshwater ecosystems across the Lublin province. Parasite genomic DNA was extracted from samples using a modified alkali wash and a heat-lysis method and identification of the Cryptosporidium species was performed at the 18SSU rRNA and COWP loci. RESULTS: The presence of Cryptosporidium DNA was only detected in one sample of intestinal scraping collected from a red-eared slider. A phylogenetic analysis of a 18SSU rRNA gene fragment showed 100% sequence identity between the C. parvum strain isolated from the turtle and other C. parvum strains previously detected in cattle from the Lublin province. CONCLUSIONS: There was no clinical evidence that the red-eared slider turtle was truly infected rather than being merely a mechanical parasite carrier. Sporadic detection of this protozoan parasite in the studied population of IAS turtles could be associated with low natural occurrence of Cryptosporidium infections in this animal species. The results provide evidence for possible transmission of zoonotic Cryptosporidium species by IAS turtles. |
format | Online Article Text |
id | pubmed-7427710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-74277102020-08-24 Detection of Cryptosporidium parvum in a Red-Eared Slider Turtle (Trachemys scripta elegans), a Noted Invasive Alien Species, Captured in a Rural Aquatic Ecosystem in Eastern Poland Rzeżutka, Artur Kaupke, Agnieszka Gorzkowski, Bartłomiej Acta Parasitol Short Communication PURPOSE: Little is known about cryptosporidiosis in turtles of invasive alien species (IAS) inhabiting European bodies of fresh water. In this article, we report an occurrence of Cryptosporidium parvum in a red-eared slider turtle (Trachemys scripta elegans) captured in a rural aquatic ecosystem in eastern Poland. METHODS: A pair of samples consisting of feces and scrapings of intestinal mucosa (taken during necropsy) were collected from 104 animals representing the four IAS turtle species red-eared slider, yellow-bellied slider, false map and Cumberland slider. The animals were trapped in running and standing freshwater ecosystems across the Lublin province. Parasite genomic DNA was extracted from samples using a modified alkali wash and a heat-lysis method and identification of the Cryptosporidium species was performed at the 18SSU rRNA and COWP loci. RESULTS: The presence of Cryptosporidium DNA was only detected in one sample of intestinal scraping collected from a red-eared slider. A phylogenetic analysis of a 18SSU rRNA gene fragment showed 100% sequence identity between the C. parvum strain isolated from the turtle and other C. parvum strains previously detected in cattle from the Lublin province. CONCLUSIONS: There was no clinical evidence that the red-eared slider turtle was truly infected rather than being merely a mechanical parasite carrier. Sporadic detection of this protozoan parasite in the studied population of IAS turtles could be associated with low natural occurrence of Cryptosporidium infections in this animal species. The results provide evidence for possible transmission of zoonotic Cryptosporidium species by IAS turtles. Springer International Publishing 2020-03-05 2020 /pmc/articles/PMC7427710/ /pubmed/32141020 http://dx.doi.org/10.2478/s11686-020-00180-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Short Communication Rzeżutka, Artur Kaupke, Agnieszka Gorzkowski, Bartłomiej Detection of Cryptosporidium parvum in a Red-Eared Slider Turtle (Trachemys scripta elegans), a Noted Invasive Alien Species, Captured in a Rural Aquatic Ecosystem in Eastern Poland |
title | Detection of Cryptosporidium parvum in a Red-Eared Slider Turtle (Trachemys scripta elegans), a Noted Invasive Alien Species, Captured in a Rural Aquatic Ecosystem in Eastern Poland |
title_full | Detection of Cryptosporidium parvum in a Red-Eared Slider Turtle (Trachemys scripta elegans), a Noted Invasive Alien Species, Captured in a Rural Aquatic Ecosystem in Eastern Poland |
title_fullStr | Detection of Cryptosporidium parvum in a Red-Eared Slider Turtle (Trachemys scripta elegans), a Noted Invasive Alien Species, Captured in a Rural Aquatic Ecosystem in Eastern Poland |
title_full_unstemmed | Detection of Cryptosporidium parvum in a Red-Eared Slider Turtle (Trachemys scripta elegans), a Noted Invasive Alien Species, Captured in a Rural Aquatic Ecosystem in Eastern Poland |
title_short | Detection of Cryptosporidium parvum in a Red-Eared Slider Turtle (Trachemys scripta elegans), a Noted Invasive Alien Species, Captured in a Rural Aquatic Ecosystem in Eastern Poland |
title_sort | detection of cryptosporidium parvum in a red-eared slider turtle (trachemys scripta elegans), a noted invasive alien species, captured in a rural aquatic ecosystem in eastern poland |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427710/ https://www.ncbi.nlm.nih.gov/pubmed/32141020 http://dx.doi.org/10.2478/s11686-020-00180-8 |
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