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Using singular perturbation theory to determine kinetic parameters in a non-standard coupled enzyme assay

We investigate how to characterize the kinetic parameters of an aminotransaminase using a non-standard coupled (or auxiliary) enzyme assay, where the peculiarity arises for two reasons. First, one of the products of the auxiliary enzyme is a substrate for the primary enzyme and, second, we explicitl...

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Detalles Bibliográficos
Autores principales: Dalwadi, Mohit P., Orol, Diego, Walter, Frederik, Minton, Nigel P., King, John R., Kovács, Katalin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427744/
https://www.ncbi.nlm.nih.gov/pubmed/32761360
http://dx.doi.org/10.1007/s00285-020-01524-8