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Using singular perturbation theory to determine kinetic parameters in a non-standard coupled enzyme assay
We investigate how to characterize the kinetic parameters of an aminotransaminase using a non-standard coupled (or auxiliary) enzyme assay, where the peculiarity arises for two reasons. First, one of the products of the auxiliary enzyme is a substrate for the primary enzyme and, second, we explicitl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427744/ https://www.ncbi.nlm.nih.gov/pubmed/32761360 http://dx.doi.org/10.1007/s00285-020-01524-8 |