Toward a translationally independent RNA-based synthetic oscillator using deactivated CRISPR-Cas

In synthetic circuits, CRISPR-Cas systems have been used effectively for endpoint changes from an initial state to a final state, such as in logic gates. Here, we use deactivated Cas9 (dCas9) and deactivated Cas12a (dCas12a) to construct dynamic RNA ring oscillators that cycle continuously between s...

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Detalles Bibliográficos
Autores principales: Kuo, James, Yuan, Ruoshi, Sánchez, Carlos, Paulsson, Johan, Silver, Pamela A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430638/
https://www.ncbi.nlm.nih.gov/pubmed/32609820
http://dx.doi.org/10.1093/nar/gkaa557

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430638/
https://www.ncbi.nlm.nih.gov/pubmed/32609820
http://dx.doi.org/10.1093/nar/gkaa557

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