Detection and quantification of glycosylated queuosine modified tRNAs by acid denaturing and APB gels

Queuosine (Q) is a conserved tRNA modification in bacteria and eukaryotes. Eukaryotic Q-tRNA modification occurs through replacing the guanine base with the scavenged metabolite queuine at the wobble position of tRNAs with G(34)U(35)N(36) anticodon (Tyr, His, Asn, Asp) by the QTRT1/QTRT2 heterodimer...

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Detalles Bibliográficos
Autores principales: Zhang, Wen, Xu, Ruyi, Matuszek, Żaneta, Cai, Zhen, Pan, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430669/
https://www.ncbi.nlm.nih.gov/pubmed/32439717
http://dx.doi.org/10.1261/rna.075556.120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430669/
https://www.ncbi.nlm.nih.gov/pubmed/32439717
http://dx.doi.org/10.1261/rna.075556.120

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