Cargando…

Detection and quantification of glycosylated queuosine modified tRNAs by acid denaturing and APB gels

Queuosine (Q) is a conserved tRNA modification in bacteria and eukaryotes. Eukaryotic Q-tRNA modification occurs through replacing the guanine base with the scavenged metabolite queuine at the wobble position of tRNAs with G(34)U(35)N(36) anticodon (Tyr, His, Asn, Asp) by the QTRT1/QTRT2 heterodimer...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhang, Wen, Xu, Ruyi, Matuszek, Żaneta, Cai, Zhen, Pan, Tao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2020
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430669/ https://www.ncbi.nlm.nih.gov/pubmed/32439717 http://dx.doi.org/10.1261/rna.075556.120

Ejemplares similares

Queuosine modification protects cognate tRNAs against ribonuclease cleavage
por: Wang, Xiaoyun, et al.
Publicado: (2018)

Detection of queuosine and queuosine precursors in tRNAs by direct RNA sequencing
por: Sun, Yu, et al.
Publicado: (2023)

Queuosine‐modified tRNAs confer nutritional control of protein translation
por: Tuorto, Francesca, et al.
Publicado: (2018)

Preferential import of queuosine-modified tRNAs into Trypanosoma brucei mitochondrion is critical for organellar protein synthesis
por: Kulkarni, Sneha, et al.
Publicado: (2021)

Impact of queuosine modification of endogenous E. coli tRNAs on sense codon reassignment
por: Tittle, Jillyn M., et al.
Publicado: (2022)

Quantification of protein-protein interactions in highly denatured whey and potato protein gels
por: Tanger, Caren, et al.
Publicado: (2021)

tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors
por: Zhang, Jilei, et al.
Publicado: (2020)

OTTER, a new method for quantifying absolute amounts of tRNAs
por: Nagai, Akihisa, et al.
Publicado: (2021)

Analysis of queuosine and 2-thio tRNA modifications by high throughput sequencing
por: Katanski, Christopher D, et al.
Publicado: (2022)

Flexizyme-catalyzed synthesis of 3′-aminoacyl-NH-tRNAs
por: Katoh, Takayuki, et al.
Publicado: (2019)

A method to enrich polypeptidyl-tRNAs to capture snapshots of translation in the cell
por: Yamakawa, Ayako, et al.
Publicado: (2023)

Optimization of the hybridization-based method for purification of thermostable tRNAs in the presence of tetraalkylammonium salts
por: Yokogawa, Takashi, et al.
Publicado: (2010)

Chimeric tRNAs as tools to induce proteome damage and identify components of stress responses
por: Geslain, Renaud, et al.
Publicado: (2010)

Monitoring the 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) modification in eukaryotic tRNAs via the γ-toxin endonuclease
por: Lentini, Jenna M., et al.
Publicado: (2018)

Retrograde tRNAs
por: Tuma, Rabiya S.
Publicado: (2005)

Synthesis and Structure Elucidation of Glutamyl-Queuosine
por: Pichler, Alexander, et al.
Publicado: (2023)

To be or not to be modified: Miscellaneous aspects influencing nucleotide modifications in tRNAs
por: Barraud, Pierre, et al.
Publicado: (2019)

tRNAs as Antibiotic Targets
por: Chopra, Shaileja, et al.
Publicado: (2014)

tRNAs taking charge
por: Cruz, Jonathan W., et al.
Publicado: (2015)

Hijacking tRNAs From Translation: Regulatory Functions of tRNAs in Mammalian Cell Physiology
por: Avcilar-Kucukgoze, Irem, et al.
Publicado: (2020)

Next-generation sequencing of dsRNA is greatly improved by treatment with the inexpensive denaturing reagent DMSO
por: Wilcox, Alexander H., et al.
Publicado: (2019)

A polyhedron made of tRNAs
por: Severcan, Isil, et al.
Publicado: (2010)

Genomic organization of eukaryotic tRNAs
por: Bermudez-Santana, Clara, et al.
Publicado: (2010)

tRNAs as regulators of biological processes
por: Raina, Medha, et al.
Publicado: (2014)

Exploiting tRNAs to Boost Virulence
por: Albers, Suki, et al.
Publicado: (2016)

Surveillance and Cleavage of Eukaryotic tRNAs
por: Megel, Cyrille, et al.
Publicado: (2015)

Repurposing tRNAs for nonsense suppression
por: Albers, Suki, et al.
Publicado: (2021)

In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system
por: Taira, Hikaru, et al.
Publicado: (2006)

Reconstruction of full-length circular RNAs enables isoform-level quantification
por: Zheng, Yi, et al.
Publicado: (2019)

SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs
por: Rabin, Avigayel, et al.
Publicado: (2021)

TET2 chemically modifies tRNAs and regulates tRNA fragment levels
por: He, Chongsheng, et al.
Publicado: (2020)

Undetected antisense tRNAs in mitochondrial genomes?
por: Seligmann, Hervé
Publicado: (2010)

Armless mitochondrial tRNAs in Enoplea (Nematoda)
por: Jühling, Frank, et al.
Publicado: (2012)

Rational Design of Aptamer-Tagged tRNAs
por: Mukai, Takahito
Publicado: (2020)

Biosynthesis and Degradation of Sulfur Modifications in tRNAs
por: Shigi, Naoki
Publicado: (2021)

Phylogeny and evolution of chloroplast tRNAs in Adoxaceae
por: Zhong, Qiu‐Yi, et al.
Publicado: (2021)

A neutral pH thermal hydrolysis method for quantification of structured RNAs
por: Wilson, Stephen C., et al.
Publicado: (2014)

Bacterial noncoding Y RNAs are widespread and mimic tRNAs
por: Chen, Xinguo, et al.
Publicado: (2014)

Syntheses of (15)N-labeled pre-queuosine nucleobase derivatives
por: Levic, Jasmin, et al.
Publicado: (2014)

Cannabidiol sensitizes TRPV2 channels to activation by 2-APB
por: Gochman, Aaron, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_4k7nejdr6h0gde0nthr47kpr0e