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Chemical crosslinking enhances RNA immunoprecipitation for efficient identification of binding sites of proteins that photo-crosslink poorly with RNA
In eukaryotic cells, proteins that associate with RNA regulate its activity to control cellular function. To fully illuminate the basis of RNA function, it is essential to identify such RNA-associated proteins, their mode of action on RNA, and their preferred RNA targets and binding sites. By analyz...
Autores principales: | Patton, Robert D., Sanjeev, Manu, Woodward, Lauren A., Mabin, Justin W., Bundschuh, Ralf, Singh, Guramrit |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430673/ https://www.ncbi.nlm.nih.gov/pubmed/32467309 http://dx.doi.org/10.1261/rna.074856.120 |
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