CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs

Precise genome editing using CRISPR-Cas9 is a promising therapeutic avenue for genetic diseases, although off-target editing remains a significant safety concern. Guide RNAs shorter than 16 nucleotides in length effectively recruit Cas9 to complementary sites in the genome but do not permit Cas9 nuc...

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Detalles Bibliográficos
Autores principales: Coelho, Matthew A., De Braekeleer, Etienne, Firth, Mike, Bista, Michal, Lukasiak, Sebastian, Cuomo, Maria Emanuela, Taylor, Benjamin J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431537/
https://www.ncbi.nlm.nih.gov/pubmed/32807781
http://dx.doi.org/10.1038/s41467-020-17952-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431537/
https://www.ncbi.nlm.nih.gov/pubmed/32807781
http://dx.doi.org/10.1038/s41467-020-17952-5

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