Plasmid-based complementation of large deletions in Phaeodactylum tricornutum biosynthetic genes generated by Cas9 editing

The model diatom Phaeodactylum tricornutum is an attractive candidate for synthetic biology applications. Development of auxotrophic strains of P. tricornutum would provide alternative selective markers to commonly used antibiotic resistance genes. Here, using CRISPR/Cas9, we show successful editing...

Descripción completa

Detalles Bibliográficos
Autores principales: Slattery, Samuel S., Wang, Helen, Giguere, Daniel J., Kocsis, Csanad, Urquhart, Bradley L., Karas, Bogumil J., Edgell, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431573/
https://www.ncbi.nlm.nih.gov/pubmed/32807825
http://dx.doi.org/10.1038/s41598-020-70769-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431573/
https://www.ncbi.nlm.nih.gov/pubmed/32807825
http://dx.doi.org/10.1038/s41598-020-70769-6

Ejemplares similares