Cargando…
Melatonin Suppresses Renal Cortical Fibrosis by Inhibiting Cytoskeleton Reorganization and Mitochondrial Dysfunction through Regulation of miR-4516
Renal fibrosis, a major risk factor for kidney failure, can lead to chronic kidney disease (CKD) and is caused by cytoskeleton reorganization and mitochondrial dysfunction. In this study, we investigated the potential of melatonin treatment to reduce renal fibrosis by recovering the cytoskeleton reo...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432329/ https://www.ncbi.nlm.nih.gov/pubmed/32727098 http://dx.doi.org/10.3390/ijms21155323 |
_version_ | 1783571773041672192 |
---|---|
author | Yoon, Yeo Min Go, Gyeongyun Yun, Chul Won Lim, Ji Ho Lee, Jun Hee Lee, Sang Hun |
author_facet | Yoon, Yeo Min Go, Gyeongyun Yun, Chul Won Lim, Ji Ho Lee, Jun Hee Lee, Sang Hun |
author_sort | Yoon, Yeo Min |
collection | PubMed |
description | Renal fibrosis, a major risk factor for kidney failure, can lead to chronic kidney disease (CKD) and is caused by cytoskeleton reorganization and mitochondrial dysfunction. In this study, we investigated the potential of melatonin treatment to reduce renal fibrosis by recovering the cytoskeleton reorganization and mitochondrial dysfunction. We found that miR-4516 expression was downregulated in the renal cortex of CKD mice and P-cresol-treated TH1 cells. Decreased miR-4516 expression stimulated cytoskeleton reorganization and mitochondrial dysfunction, and induced renal fibrosis. Melatonin treatment suppressed fibrosis by inhibiting cytoskeleton reorganization and restoring mitochondrial function via increased miR-4516 expression. More specifically, melatonin treatment increased miR-4516 expression while decreasing ITGA9 expression, thereby inhibiting cytoskeleton reorganization. In addition, increased expression of miR-4516 by melatonin treatment reduced ROS formation and restored mitochondrial function. These findings suggest that melatonin may be a promising treatment for patients with CKD having renal fibrosis. Moreover, regulation of miR-4516 expression may be a novel strategy for the treatment of renal fibrosis. |
format | Online Article Text |
id | pubmed-7432329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74323292020-08-24 Melatonin Suppresses Renal Cortical Fibrosis by Inhibiting Cytoskeleton Reorganization and Mitochondrial Dysfunction through Regulation of miR-4516 Yoon, Yeo Min Go, Gyeongyun Yun, Chul Won Lim, Ji Ho Lee, Jun Hee Lee, Sang Hun Int J Mol Sci Article Renal fibrosis, a major risk factor for kidney failure, can lead to chronic kidney disease (CKD) and is caused by cytoskeleton reorganization and mitochondrial dysfunction. In this study, we investigated the potential of melatonin treatment to reduce renal fibrosis by recovering the cytoskeleton reorganization and mitochondrial dysfunction. We found that miR-4516 expression was downregulated in the renal cortex of CKD mice and P-cresol-treated TH1 cells. Decreased miR-4516 expression stimulated cytoskeleton reorganization and mitochondrial dysfunction, and induced renal fibrosis. Melatonin treatment suppressed fibrosis by inhibiting cytoskeleton reorganization and restoring mitochondrial function via increased miR-4516 expression. More specifically, melatonin treatment increased miR-4516 expression while decreasing ITGA9 expression, thereby inhibiting cytoskeleton reorganization. In addition, increased expression of miR-4516 by melatonin treatment reduced ROS formation and restored mitochondrial function. These findings suggest that melatonin may be a promising treatment for patients with CKD having renal fibrosis. Moreover, regulation of miR-4516 expression may be a novel strategy for the treatment of renal fibrosis. MDPI 2020-07-27 /pmc/articles/PMC7432329/ /pubmed/32727098 http://dx.doi.org/10.3390/ijms21155323 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yoon, Yeo Min Go, Gyeongyun Yun, Chul Won Lim, Ji Ho Lee, Jun Hee Lee, Sang Hun Melatonin Suppresses Renal Cortical Fibrosis by Inhibiting Cytoskeleton Reorganization and Mitochondrial Dysfunction through Regulation of miR-4516 |
title | Melatonin Suppresses Renal Cortical Fibrosis by Inhibiting Cytoskeleton Reorganization and Mitochondrial Dysfunction through Regulation of miR-4516 |
title_full | Melatonin Suppresses Renal Cortical Fibrosis by Inhibiting Cytoskeleton Reorganization and Mitochondrial Dysfunction through Regulation of miR-4516 |
title_fullStr | Melatonin Suppresses Renal Cortical Fibrosis by Inhibiting Cytoskeleton Reorganization and Mitochondrial Dysfunction through Regulation of miR-4516 |
title_full_unstemmed | Melatonin Suppresses Renal Cortical Fibrosis by Inhibiting Cytoskeleton Reorganization and Mitochondrial Dysfunction through Regulation of miR-4516 |
title_short | Melatonin Suppresses Renal Cortical Fibrosis by Inhibiting Cytoskeleton Reorganization and Mitochondrial Dysfunction through Regulation of miR-4516 |
title_sort | melatonin suppresses renal cortical fibrosis by inhibiting cytoskeleton reorganization and mitochondrial dysfunction through regulation of mir-4516 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432329/ https://www.ncbi.nlm.nih.gov/pubmed/32727098 http://dx.doi.org/10.3390/ijms21155323 |
work_keys_str_mv | AT yoonyeomin melatoninsuppressesrenalcorticalfibrosisbyinhibitingcytoskeletonreorganizationandmitochondrialdysfunctionthroughregulationofmir4516 AT gogyeongyun melatoninsuppressesrenalcorticalfibrosisbyinhibitingcytoskeletonreorganizationandmitochondrialdysfunctionthroughregulationofmir4516 AT yunchulwon melatoninsuppressesrenalcorticalfibrosisbyinhibitingcytoskeletonreorganizationandmitochondrialdysfunctionthroughregulationofmir4516 AT limjiho melatoninsuppressesrenalcorticalfibrosisbyinhibitingcytoskeletonreorganizationandmitochondrialdysfunctionthroughregulationofmir4516 AT leejunhee melatoninsuppressesrenalcorticalfibrosisbyinhibitingcytoskeletonreorganizationandmitochondrialdysfunctionthroughregulationofmir4516 AT leesanghun melatoninsuppressesrenalcorticalfibrosisbyinhibitingcytoskeletonreorganizationandmitochondrialdysfunctionthroughregulationofmir4516 |