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A 4-plex Droplet Digital PCR Method for Simultaneous Quantification and Differentiation of Pathogenic and Non-pathogenic Vibrio parahaemolyticus Based on Single Intact Cells

Vibrio parahaemolyticus is a significant seafood-borne pathogen, leading to serious acute gastrointestinal diseases worldwide. In this study, a reliable 4-plex droplet digital PCR (ddPCR) was successfully established and evaluated for the simultaneous detection of V. parahaemolyticus based on tlh, t...

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Detalles Bibliográficos
Autores principales: Lei, Shuwen, Gu, Xiaokui, Xue, Wei, Rong, Zhangquan, Wang, Zhe, Chen, Song, Zhong, Qingping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7434843/
https://www.ncbi.nlm.nih.gov/pubmed/32903334
http://dx.doi.org/10.3389/fmicb.2020.01727