A Modified Pre-plating Method for High-Yield and High-Purity Muscle Stem Cell Isolation From Human/Mouse Skeletal Muscle Tissues

Primary culture of skeletal muscle stem cells (MuSCs) is indispensable to study the dynamics of muscle regeneration and homeostasis. Here we describe the modified pre-plating method for isolating MuSCs in culture with greatly improved purity, yield, and procedure time. The protocol is based on the d...

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Detalles Bibliográficos
Autores principales: Yoshioka, Kiyoshi, Kitajima, Yasuo, Okazaki, Narihiro, Chiba, Ko, Yonekura, Akihiko, Ono, Yusuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438441/
https://www.ncbi.nlm.nih.gov/pubmed/32903486
http://dx.doi.org/10.3389/fcell.2020.00793

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438441/
https://www.ncbi.nlm.nih.gov/pubmed/32903486
http://dx.doi.org/10.3389/fcell.2020.00793

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