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Structure-specific DNA recombination sites: Design, validation, and machine learning–based refinement
Recombination systems are widely used as bioengineering tools, but their sites have to be highly similar to a consensus sequence or to each other. To develop a recombination system free of these constraints, we turned toward attC sites from the bacterial integron system: single-stranded DNA hairpins...
Autores principales: | Nivina, Aleksandra, Grieb, Maj Svea, Loot, Céline, Bikard, David, Cury, Jean, Shehata, Laila, Bernardes, Juliana, Mazel, Didier |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439510/ https://www.ncbi.nlm.nih.gov/pubmed/32832653 http://dx.doi.org/10.1126/sciadv.aay2922 |
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