A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9

CRISPR technologies have overwhelmingly relied on the Streptococcus pyogenes Cas9 (SpyCas9), with its consensus NGG and less preferred NAG and NGA protospacer-adjacent motifs (PAMs). Here, we report that SpyCas9 also recognizes sequences within an N(A/C/T)GG motif. These sequences were identified on...

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Detalles Bibliográficos
Autores principales: Collias, D., Leenay, R. T., Slotkowski, R. A., Zuo, Z., Collins, S. P., McGirr, B. A., Liu, J., Beisel, C. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439565/
https://www.ncbi.nlm.nih.gov/pubmed/32832642
http://dx.doi.org/10.1126/sciadv.abb4054

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439565/
https://www.ncbi.nlm.nih.gov/pubmed/32832642
http://dx.doi.org/10.1126/sciadv.abb4054

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