Cargando…
Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi
In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, a few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). The present study aimed...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442975/ https://www.ncbi.nlm.nih.gov/pubmed/32785580 http://dx.doi.org/10.1042/BSR20200950 |
_version_ | 1783573542096338944 |
---|---|
author | de Azevedo dos Santos, Layrana Taveira, Gabriel Bonan da Silva, Marciele Souza da Silva Gebara, Rodrigo da Silva Pereira, Lídia Perales, Jonas Teixeira-Ferreira, André de Oliveira Mello, Érica de Oliveira Carvalho, André Rodrigues, Rosana Gomes, Valdirene Moreira |
author_facet | de Azevedo dos Santos, Layrana Taveira, Gabriel Bonan da Silva, Marciele Souza da Silva Gebara, Rodrigo da Silva Pereira, Lídia Perales, Jonas Teixeira-Ferreira, André de Oliveira Mello, Érica de Oliveira Carvalho, André Rodrigues, Rosana Gomes, Valdirene Moreira |
author_sort | de Azevedo dos Santos, Layrana |
collection | PubMed |
description | In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, a few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). The present study aimed to purify and characterize peptides from Capsicum chinense fruits and evaluate their inhibitory activities against different phytopathogenic fungi and also analyze the possible mechanisms of action involved in microbial inhibition. After fruit protein extraction and high-performance liquid chromatography (HPLC), different fractions were obtained, named F1 to F10. Peptides in the F4 and F5 fractions were sequenced and revealed similarity with the plant antimicrobial peptides like non-specific lipid transfer proteins and defensin-like peptide. The F4 and F5 fractions presented strong antimicrobial activity against the fungus Fusarium solani and Fusarium oxysporum, causing toxic effects on these fungi, leading to membrane permeabilization, endogenous reactive oxygen species increase, activation of metacaspase and loss of mitochondrial function. |
format | Online Article Text |
id | pubmed-7442975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74429752020-08-26 Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi de Azevedo dos Santos, Layrana Taveira, Gabriel Bonan da Silva, Marciele Souza da Silva Gebara, Rodrigo da Silva Pereira, Lídia Perales, Jonas Teixeira-Ferreira, André de Oliveira Mello, Érica de Oliveira Carvalho, André Rodrigues, Rosana Gomes, Valdirene Moreira Biosci Rep Plant Biology In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, a few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). The present study aimed to purify and characterize peptides from Capsicum chinense fruits and evaluate their inhibitory activities against different phytopathogenic fungi and also analyze the possible mechanisms of action involved in microbial inhibition. After fruit protein extraction and high-performance liquid chromatography (HPLC), different fractions were obtained, named F1 to F10. Peptides in the F4 and F5 fractions were sequenced and revealed similarity with the plant antimicrobial peptides like non-specific lipid transfer proteins and defensin-like peptide. The F4 and F5 fractions presented strong antimicrobial activity against the fungus Fusarium solani and Fusarium oxysporum, causing toxic effects on these fungi, leading to membrane permeabilization, endogenous reactive oxygen species increase, activation of metacaspase and loss of mitochondrial function. Portland Press Ltd. 2020-08-21 /pmc/articles/PMC7442975/ /pubmed/32785580 http://dx.doi.org/10.1042/BSR20200950 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
spellingShingle | Plant Biology de Azevedo dos Santos, Layrana Taveira, Gabriel Bonan da Silva, Marciele Souza da Silva Gebara, Rodrigo da Silva Pereira, Lídia Perales, Jonas Teixeira-Ferreira, André de Oliveira Mello, Érica de Oliveira Carvalho, André Rodrigues, Rosana Gomes, Valdirene Moreira Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi |
title | Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi |
title_full | Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi |
title_fullStr | Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi |
title_full_unstemmed | Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi |
title_short | Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi |
title_sort | antimicrobial peptides from capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi |
topic | Plant Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442975/ https://www.ncbi.nlm.nih.gov/pubmed/32785580 http://dx.doi.org/10.1042/BSR20200950 |
work_keys_str_mv | AT deazevedodossantoslayrana antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi AT taveiragabrielbonan antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi AT dasilvamarcielesouza antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi AT dasilvagebararodrigo antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi AT dasilvapereiralidia antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi AT peralesjonas antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi AT teixeiraferreiraandre antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi AT deoliveiramelloerica antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi AT deoliveiracarvalhoandre antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi AT rodriguesrosana antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi AT gomesvaldirenemoreira antimicrobialpeptidesfromcapsicumchinensefruitsagronomicalternativesagainstphytopathogenicfungi |