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Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi

In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, a few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). The present study aimed...

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Autores principales: de Azevedo dos Santos, Layrana, Taveira, Gabriel Bonan, da Silva, Marciele Souza, da Silva Gebara, Rodrigo, da Silva Pereira, Lídia, Perales, Jonas, Teixeira-Ferreira, André, de Oliveira Mello, Érica, de Oliveira Carvalho, André, Rodrigues, Rosana, Gomes, Valdirene Moreira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442975/
https://www.ncbi.nlm.nih.gov/pubmed/32785580
http://dx.doi.org/10.1042/BSR20200950
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author de Azevedo dos Santos, Layrana
Taveira, Gabriel Bonan
da Silva, Marciele Souza
da Silva Gebara, Rodrigo
da Silva Pereira, Lídia
Perales, Jonas
Teixeira-Ferreira, André
de Oliveira Mello, Érica
de Oliveira Carvalho, André
Rodrigues, Rosana
Gomes, Valdirene Moreira
author_facet de Azevedo dos Santos, Layrana
Taveira, Gabriel Bonan
da Silva, Marciele Souza
da Silva Gebara, Rodrigo
da Silva Pereira, Lídia
Perales, Jonas
Teixeira-Ferreira, André
de Oliveira Mello, Érica
de Oliveira Carvalho, André
Rodrigues, Rosana
Gomes, Valdirene Moreira
author_sort de Azevedo dos Santos, Layrana
collection PubMed
description In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, a few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). The present study aimed to purify and characterize peptides from Capsicum chinense fruits and evaluate their inhibitory activities against different phytopathogenic fungi and also analyze the possible mechanisms of action involved in microbial inhibition. After fruit protein extraction and high-performance liquid chromatography (HPLC), different fractions were obtained, named F1 to F10. Peptides in the F4 and F5 fractions were sequenced and revealed similarity with the plant antimicrobial peptides like non-specific lipid transfer proteins and defensin-like peptide. The F4 and F5 fractions presented strong antimicrobial activity against the fungus Fusarium solani and Fusarium oxysporum, causing toxic effects on these fungi, leading to membrane permeabilization, endogenous reactive oxygen species increase, activation of metacaspase and loss of mitochondrial function.
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spelling pubmed-74429752020-08-26 Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi de Azevedo dos Santos, Layrana Taveira, Gabriel Bonan da Silva, Marciele Souza da Silva Gebara, Rodrigo da Silva Pereira, Lídia Perales, Jonas Teixeira-Ferreira, André de Oliveira Mello, Érica de Oliveira Carvalho, André Rodrigues, Rosana Gomes, Valdirene Moreira Biosci Rep Plant Biology In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, a few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). The present study aimed to purify and characterize peptides from Capsicum chinense fruits and evaluate their inhibitory activities against different phytopathogenic fungi and also analyze the possible mechanisms of action involved in microbial inhibition. After fruit protein extraction and high-performance liquid chromatography (HPLC), different fractions were obtained, named F1 to F10. Peptides in the F4 and F5 fractions were sequenced and revealed similarity with the plant antimicrobial peptides like non-specific lipid transfer proteins and defensin-like peptide. The F4 and F5 fractions presented strong antimicrobial activity against the fungus Fusarium solani and Fusarium oxysporum, causing toxic effects on these fungi, leading to membrane permeabilization, endogenous reactive oxygen species increase, activation of metacaspase and loss of mitochondrial function. Portland Press Ltd. 2020-08-21 /pmc/articles/PMC7442975/ /pubmed/32785580 http://dx.doi.org/10.1042/BSR20200950 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
spellingShingle Plant Biology
de Azevedo dos Santos, Layrana
Taveira, Gabriel Bonan
da Silva, Marciele Souza
da Silva Gebara, Rodrigo
da Silva Pereira, Lídia
Perales, Jonas
Teixeira-Ferreira, André
de Oliveira Mello, Érica
de Oliveira Carvalho, André
Rodrigues, Rosana
Gomes, Valdirene Moreira
Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi
title Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi
title_full Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi
title_fullStr Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi
title_full_unstemmed Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi
title_short Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi
title_sort antimicrobial peptides from capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi
topic Plant Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442975/
https://www.ncbi.nlm.nih.gov/pubmed/32785580
http://dx.doi.org/10.1042/BSR20200950
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