The Petri Dish-N2B27 Culture Condition Maintains RPE Phenotype by Inhibiting Cell Proliferation and mTOR Activation

OBJECTIVE: To develop a method for the rapid isolation of rat RPE cells with high yield and maintain its epithelial state in modified culture system. METHODS: The eyeballs were incubated with dispase. The retina was isolated with RPE attached and cut into several pieces. Following a brief incubation...

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Detalles Bibliográficos
Autores principales: Lou, Hui, Lian, Chunpin, Shi, Fanjun, Chen, Liqun, Qian, Sicheng, Wang, Hui, Zhao, Xiaoyun, Ji, Xiaoyan, Zhang, Jingfa, Xu, Guoxu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443227/
https://www.ncbi.nlm.nih.gov/pubmed/32855817
http://dx.doi.org/10.1155/2020/4892978

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443227/
https://www.ncbi.nlm.nih.gov/pubmed/32855817
http://dx.doi.org/10.1155/2020/4892978

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