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The Petri Dish-N2B27 Culture Condition Maintains RPE Phenotype by Inhibiting Cell Proliferation and mTOR Activation
OBJECTIVE: To develop a method for the rapid isolation of rat RPE cells with high yield and maintain its epithelial state in modified culture system. METHODS: The eyeballs were incubated with dispase. The retina was isolated with RPE attached and cut into several pieces. Following a brief incubation...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Hindawi
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443227/ https://www.ncbi.nlm.nih.gov/pubmed/32855817 http://dx.doi.org/10.1155/2020/4892978 |