Single-strand RPA for rapid and sensitive detection of SARS-CoV-2 RNA

We report the single-strand Recombinase Polymerase Amplification (ssRPA) method, which merges the fast, isothermal amplification of RPA with subsequent rapid conversion of the double-strand DNA amplicon to single strands, and hence enables facile hybridization-based, high-specificity readout. We dem...

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Detalles Bibliográficos
Autores principales: Kim, Youngeun, Yaseen, Adam B., Kishi, Jocelyn Y., Hong, Fan, Saka, Sinem K., Sheng, Kuanwei, Gopalkrishnan, Nikhil, Schaus, Thomas E., Yin, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444299/
https://www.ncbi.nlm.nih.gov/pubmed/32839783
http://dx.doi.org/10.1101/2020.08.17.20177006

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444299/
https://www.ncbi.nlm.nih.gov/pubmed/32839783
http://dx.doi.org/10.1101/2020.08.17.20177006

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